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MultiClamp 700B Amplifier, Patch Clamp, Elektrofiziológia

For single-channel recordings, whole-cell voltage- and current-clamp recordings, intracellular sharp electrode recordings, extracellular field potential recordings in ion channel, neuroscience, nanopore, voltammetry studies

A microelectrode amplifier that combines leading edge electronics design and a wealth of features and functions with an intuitive user interface that simplifies the creation of complex protocols.

ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!

Leírás

The MultiClamp™ 700B Microelectrode Amplifier is a versatile, computer-controlled microelectrode amplifier designed for patch voltage-clamp (resistive-feedback, four ranges) or high-speed current clamp (voltage-follower, three ranges) recording within the same headstage. Many automated functions and effective signal conditioning. Ideal for many applications, including high-speed current clamp (sharp-electrode or field potentials), patch clamp (whole-cell, macro-patch, or excised patch), voltammetry/amperometry, ion-selective measurements and bilayer recordings. Add two more optional headstage (voltage-follower) inputs for a four-point recording system, ideal for complex synaptic recordings. Major new features of the MultiClamp 700B Amplifier include Internal Automatic Mode switching, triggered by external signals or the recording itself; Oscillation Suppression for better cell protection; and Slow Current Injection to compensate for drifting potentials.

Support of up to four headstages makes the MultiClamp 700B System four amplifiers in one.
Software control of all amplifier settings for automated optimization features, reduction of manipulation steps, and convenient, reliable telegraphing.
Quick Select Feature recalls up to three saved amplifier configurations for fast, reproducible application switching.
Optional SoftPanel allows amplifier control using knobs and buttons for a more conventional feel to amplifier control.
Integrated seal test in voltage- and current-clamp mode for easy monitoring of membrane and cell health parameters.
Dual Command Potentials add flexibility by processing input signals from two different sources.
Selectable command sensitivity for flexible control of the output waveform.
Pipette offset compensation with auto-mode quickly removes signal offsets.
Automatic pipette capacitance compensation requires only the push of a button to neutralize the contribution of the glass pipette and the pipette holder to the capacitance of the circuitry.
Series resistance compensation: Prediction helps achieve the desired command level faster by transiently supercharging the membrane potential.
Series resistance compensation: Correction improves the bandwidth of the recording by eliminating the error introduced by the voltage drop across the series resistance.
Automatic cell capacitance compensation corrects for the capacitance of the cell membrane in whole-cell recordings with a single mouse click.
Automatic mode switching enables you to control the transition between voltage and current clamp by an external trigger or depending on the recorded signal.
Automatic oscillation detection prevents damage to a cell when electrical feedback occurs.
Leak subtraction for automatic correction of leak currents with a smart, software-based algorithm.
Selectable output gain with 11 settings to scale the output signal to the desired level.
Audio monitor for acoustic tracking of the signal while you are busy looking down a microscope.
Fine-tunable 4-pole bessel and butterworth low-pass filters act as anti-aliasing filters and can be used to pre-condition the output signal.

Műszaki specifikációk

General Specifications
Dimensions (in.)	3.5 (H)x 19 (W) x 12 (D)
Dimensions (cm)	8.9 H) x 48.3 (W) x 30.5 (D)
Weight	10 lbs. (4.54 kg)
Headstage (in.)	0.875 (H) x 1.625(W) x 2.3125 (D)
Headstage (cm)	2.0 (H) x 4.0 (W) x 8.4 (D)
Channels	2 (sharing a common ground)
Communications	USB 1 Type B female ports
Rack use	Standard 19" rack-mount (2U) with handles
Benchtop use	Bayonet feet
Power	85-260 VAC 50-60 Hz, 30 W (max)
Safety	CE marking (Conformité Européenne)

Applikációk

Basic Ion Channel Research

Ion channels are pore proteins that are involved in a large variety of physiological processes. Basic research in the ion channel field furthers the understanding of ion channel function as well as their roles within cellular pathways and processes. Single-channel research helps with elucidating the mechanisms underlying ion channel function on a single-molecule level. Whole-cell and perforated patch clamp recordings are typically used to investigate the action of modulators on entire populations of ion channels in the membrane of a cell.

Cell Pathway Analysis

Ion channels are involved in many cell pathways and understanding the function of ion channels in response to changes in membrane potential or the presence or absence of other molecules is important in order to understand exactly how ion channels participate in normal and abnormal biological processes such as cell differentiation and migration, disease states, neuronal communications, etc.

Disease Research

Ion channels play a role in many diseases, including hypertension, cardiac arrhythmias, gastrointestinal, immune and neuromuscular disorders, pathological pain and cancer. By understanding the exact role that ion channels play in a particular disease, researchers might be able to find a way to affect the ion channel in such a way as to alter the course of the disease.

Safety Assessment

A critical part of drug discovery involves safety assessments, such as hERG testing, in order to eliminate any compounds with potential safety concerns. Conventional patch clamp has played an important role in collecting evidence for the absence of side effects before submission of a drug candidate to regulatory authorities. Even if automated electrophysiology has shifted safety testing to earlier stages in the drug discovery process, follow-up with conventional patch clamp is an essential step in the workflow.

Tartozékok, kiszerelések

The MultiClamp 700B comes with two identical but independent CV-7B standard headstages, each of which contains both current-to-voltage and voltage-following circuitry. This design allows the user to rapidly switch between patch clamp recording and true high-speed current clamp recording. Thus, with two headstages, the MultiClamp 700B can perform the function of two patch clamps, two current clamps, or a combination of patch and current clamp amplifiers. Furthermore, two optional voltage-follower headstages (HS-2 type) can be connected to auxiliary inputs to allow third and fourth-point voltage recording.

HS-2 and HS-2A unity-gain headstages

The HS-2 and HS-2A headstages are used with Axoclamp 2 and GeneClamp amplifiers. They are all unity-gain voltage recording headstages but they come in a variety of different current-passing gains for applications as diverse as extracellular recording, bath-potential recording, ion-sensitive recording, ionophoresis and intracellular recording from small or large cells. For ultra-high impedance electrodes, special circuitry inside the headstage prevents any DC current from leaking into the input through the capacitance neutralization circuit.

HS-2-x0.0001MU
HS-2-x0.01MU
HS-2A-x0.1LU
HS-2A-x1LU
HS-2A-x1MGU
HS-2A-x10MGU
HS-2A-x100MGU
VG-2A-x100 (virtual ground)

The CV-7B was designed for low noise and flexible recording features. However, some specialized applications require even more flexibility. For example, the rather large membrane capacitance in bilayer recording demands greater capacitance compensation. The optional CV-7B/BL headstage was developed for this purpose. Another optional headstage, the CV-7B/EC, was designed for large (± 2 V) commands required during electrochemistry recording (amperometry, voltammetry).

Each of the two standard CV-7B headstages can be replaced with a headstage for a specialized application. Available headstages are,

CV-7B/BL for lipid bilayer recordings
CV-7B/EC for electrochemistry recordings (amperometry, voltammetry)

MultiClamp 700B Headstages
DESCRIPTION	DETAILS	PART NUMBER
MultiClamp 700B Headstage CV-7B	Patch-clamp headstage	1-CV-7B
MultiClamp 700B Headstage CV-7B/BL	Bilayer headstage	1-CV-7B/BL
MultiClamp 700B Headstage CV-7B/EC	Electrochemistry headstage	1-CV-7B/EC

MultiClamp Commander

Instead of the traditional front panel knobs and switches, the MultiClamp 700B Amplifier is controlled by the MultiClamp 700B Commander, a software program that runs on a host computer (PC and Macintosh; see System Requirements) and communicates with the amplifier via a USB interface. This control interface reports resistance, voltage, and current measurements and provides automation of Bridge Balance, Pipette Offset, and Pipette Capacitance Neutralization for improved ease of use. Amplifier settings such as gain, filter frequency, whole-cell capacitance, recording mode, and input/output scale factors are automatically telegraphed to the data acquisition software via "messaging" through the computer operating system. Quick Select provides easy access to stored preset configurations that apply to particular applications.

In addition, MultiClamp Commander has "smart" features that protect cells from potentially damaging signal oscillations and automatic mode changes based on internal signal thresholds or externally applied signals. Amplifier settings such as gain, filter frequency, recording mode, and input/output scale factors are automatically telegraphed to thepCLAMP 10 Data Acquisition Software via the USB connection. This ensures that the amplifier settings are accurately and conveniently stored in the recorded data files.

To obtain the installer for a more in-depth look at MultiClamp 700B Commander Software, please go to our Customer Support page.

In combination with a Digidata® 1550 Data Acquisition System and pCLAMP™ Software, the MultiClamp 700B Amplifier forms the core of a setup like those used in many electrophysiology labs around the world. Computer control of the amplifier ensures correct scaling and logging of experimental parameters. The Clampex Data Acquisition Module, which is part of the pCLAMP Software, makes optimal use of the features of the MultiClamp 700B Amplifier.

Developer Support

The MultiClamp Software Development Kit provides the tools for programmers who wish to develop software that is capable of collecting information about the state of the MultiClamp 700B or 700A Amplifier (i.e., telegraph settings). This will be useful for Microsoft Windows software development, since the applications will communicate via a registered messaging interface.

The latest version of MultiClamp 700 Commander automatically installs the files required for 3rd party support. Browse the "moleculardevicesmulticlamp-commander" directory to locate the files.

Note: Although we are committed to the integrity of this SDK, Molecular Devices will not provide direct technical support for writing programs using the SDK.

Videók, ismertetők

Multiclamp 700B ismertető

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