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Termékek
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Reagensek
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Promega élettudományi reagensek
- Fehérjeexpresszió
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Fehérjeexpresszió |
Express Protein in Hours, Not Days!
Choose from an extensive range of eukaryotic cell-free protein expression systems. Kits optimized for in vitro transcription and translation of plasmid DNA, RNA or PCR DNA are available.
RNA-based Rabbit Reticulocyte Lysate Systems can be used for investigating transcriptional/translational control and the replication of RNA-based viruses. Protein is expressed in these systems by addition of in vitro-transcribed RNA to the lysate.
Study protein trafficking in live cells, perform multicolor live and fixed-cell imaging...all with a single HaloTag genetic construct.
Choose from an extensive range of eukaryotic cell-free protein expression systems. Kits optimized for in vitro transcription and translation of plasmid DNA, RNA or PCR DNA are available.
RNA-based Rabbit Reticulocyte Lysate Systems can be used for investigating transcriptional/translational control and the replication of RNA-based viruses. Protein is expressed in these systems by addition of in vitro-transcribed RNA to the lysate.
Study protein trafficking in live cells, perform multicolor live and fixed-cell imaging...all with a single HaloTag genetic construct.
ÁRAJÁNLATOT, INFORMÁCIÓT KÉREK!
Eukaryotic Cell-Free Protein Expression
Use plasmid or PCR DNA as templates to express full-length, soluble, functional protein in 1-4 hours with the TnT® cell-free systems. No requirement to reclone; use standard vectors containing either a T7, T3 or SP6 promoter.
TnT® SP6 High-Yield Wheat Germ System
Express up to 100µg of soluble, functional, full-length protein in as little as 60 minutes directly from plasmid DNA.
TnT® Quick Systems
Add plasmid DNA directly to the TnT® Master Mix to express protein quickly in this eukaryotic-based system. No purification required after expression—use directly for protein interaction experiments.
TnT® T7 Insect Cell Extract
A convenient alternative to resource and time constrained cell-based baculovirus expression.
TnT® T7 Quick for PCR DNA
Cell-Based Protein Expression
Convenient mammalian systems and competent cells designed for easy, controlled protein expression.
Single Step KRX Competent Cells
Efficient transformation and regulated protein expression. Ideal for rapidly generating new clones for recombinant protein expression.
Regulated Mammalian Expression System
Allows high levels of controlled induction with low basal protein expression, and gives you the convenience of a rapid and sensitive on/off switch for protein expression.
FastBreak™ Cell Lysis Reagent
FastBreak™ Cell Lysis Reagent is designed for the efficient, gentle lysis of E. coli cultures without the need for centrifugation or mechanical cell disruption. The reagent is provided as a 10X concentrate. Add the reagent directly to E. coli cultures. Following a brief incubation, the cells are disrupted, and the protein of interest is released.
Analysis of Translational Regulation
Rabbit Reticulocyte Lysate Translation Systems for the identification of mRNA species, characterization of their protein products and the investigation of viral replication and translational control.
Rabbit Reticulocyte Lysate System, Nuclease Treated
Rabbit Reticulocyte Lysate is prepared from New Zealand white rabbits. After the reticulocytes are lysed, the extract is treated with micrococcal nuclease to destroy endogenous mRNA. The lysate contains the cellular components necessary for protein synthesis.
RiboMAX™ Large Scale RNA Production Systems
RiboMAX™ Large Scale RNA Production Systems consistently produce 2–5mg/ml of RNA in a 1ml reaction. RNAs synthesized with the RiboMAX™ System perform better for in vitro translation in rabbit reticulocyte translation systems than RNA synthesized by standard methods.
Flexi® Rabbit Reticulocyte Lysate System
The Flexi® Rabbit Reticulocyte Lysate System allows translation reactions to be optimized for a wide range of parameters, including Mg2+ and K+ concentrations and the choice of adding DTT. To help optimize Mg2+ for a specific message, the endogenous Mg2+concentration of each lysate batch is stated in the product information included with this product.
Prokaryotic Cell-Free Protein Expression
Express up to 500µg/ml protein directly from circular plasmid DNA using a cell-free E.coli-based system. No requirement for special equipment. Kits also available for linear or PCR DNA templates.
S30 T7 High-Yield System
Express up to 500µg/ml protein directly from plasmid DNA in only 1 hour with this E. coli-based system.
T7 S30 System for Circular Templates
To express protein, add plasmid DNA containing a T7 promoter and a ribosomal binding site to the S30 extract. No purification required after expression. Use directly for proteomics related experiments.
S30 Extract System for Linear Templates
Express protein quicky and easily from PCR, DNA fragments, ligated overlapping oligonucleotides, in vitro generated RNA, or prokaryotic RNA.
Protein Labeling and Detection
Cell imaging, in vivo imaging and protein localization systems. Fluorescent and colorimetric labeling systems for immunoblotting.
ECL Western Blotting System
A sensitive, non-radioactive, enhanced luminol-based chemiluminescent substrate for detection of horseradish peroxidase on immunoblots. Ready to use. No optimization required.
Antibody Conjugates
Our high-quality, affinity-purified HRP- and Alkaline Phosphase-conjugated antibodies for detection of bound primary antibodies in Western blot and ELISA applications.
Fluorescent Labeling
With the FluoroTect™ Green System, you can incorporate fluorescently labeled lysines during cell-free expression and detect labeled proteins directly in the gel.
Colorimetric and Chemiluminescent Labeling Systems
Protein Deamidation Detection
The ISOQUANT® Isoaspartate Detection Kit offers a safer alternative to radioactive methods for protein deamidation testing.
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