xGen Custom Amplicon Panels are custom-designed primer pools to create multiple overlapping amplicons for specific genetic targets in research studies (Figure 1). Panels can multiplex hundreds to thousands of targets in a single tube using a simple 2-hour workflow (Figure 2). Compatible with cell-free DNA and FFPE (formalin-fixed paraffin embedded) research samples starting with as little as 10 ng input or pathogen RNA/DNA in the picogram range. For a listing of the specifications for custom panels, see Table 1. If multiple libraries are being examined at the same time, the final NGS library can be normalized using the IDT proprietary xGen Normalase reagent.

The cost-effective and flexible custom design means the panel can be used for research applications such as:

• Genotyping by sequencing
• Variant detection with a limit of detection down to 1%
• Detecting germline inherited SNPs and indels
• Somatic cell variant detection
• Targeted virus or pathogen sequencing
• Genetic fingerprinting
• Liquid biopsy

Table 1. Product specifications of the xGen Custom Amplicon Panels

Simple, 2-hour workflow

From DNA to NGS library in as little as 2 hours, the workflow for the xGen Custom Amplicon Panels can be done in a single tube with minimal hands-on steps. As shown in Figure 2, the DNA sample is first amplified with the multiplex custom panel to create copies of each of your intended targets. Then the amplicons are converted into indexed libraries in a second PCR reaction with the appropriate indexing primers for your NGS instrument. When multiple libraries are pooled, IDT-proprietary xGen Normalase reagent is used to normalize the libraries for NGS analysis.