Comparison of Microplate Readers with Fluorescence Detection

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	SpectraMax M Series Multi-Mode Microplate Readers	Gemini EM Microplate Reader	Gemini XPS Microplate Reader	FLIPR Tetra High-Throughput Cellular Screening System	FlexStation 3 Multi-Mode Microplate Reader
Fluorescence	x	x	x	x	x
Absorbance	x				x
Luminescence	x+	x	x	x	x
Time-resolved fluorescence (TRF)	x+	x*	x*		x
HTRF Certified	x+				x
Fluorescence Polarization	x+				x
Wavelength range	FL 250 - 850, Abs 200 - 1000	FL 250 - 850 ex, Abs 360-850 em	FL 250 - 850 ex, 250 - 850 em	LEDs available in 360-626 nm range, Emission filters available in 400-706 nm range	FL 250 - 850 ex, 250-850 em; Abs 200-1000
Bandwidth	9/9 or 9/15+	9/9	9/9	Variable	9/15
Tunable wavelength selection/ monochromator	x	x	x		x
Endpoint	x	x	x		x
Kinetic	x	x	x	x	x
Spectral scanning	x	x	x		x
Well scanning	x	x	x		x
Reading speed (sec)	15-17+	15	15	1	17
Microplate formats	6-384	6-384	6-384	96, 384, 1536	6-384**
Integrated multi-channel pipettor					x***
Temperature control	x	x	x	x	x
Top reading	x	x	x	x	x
Bottom reading	x+	x			x
Cuvette port	x				x
Validation tools	x	x	x		x
Robotics / automation	x	x	x	x	x

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* Secondary modes
** 96- and 384-well with pipetting
*** 8- and 16-channel pipettor
+ Varies by model