Browse all SimpleStep Ignite™ ELISA kits 

Chemiluminescent ELISA kits use light-emitting reactions to offer highly sensitive and rapid detection of biomarkers.

These kits use a chemiluminescent substrate to produce light in response to antigen-antibody interactions, which allows for greater sensitivity and broader dynamic range than traditional ELISA.

Our 90-minute colorimetric SimpleStep^® ELISA kits have saved researchers around the world 82 years at the bench.

With SimpleStep Ignite™ ELISA, we’re redefining ELISAs, delivering up to 35x broader measuring range and 50x greater sensitivity with only one incubation and one wash step.

All this in an assay with an incubation time of just  30 minutes  and an overall assay time of 55 minutes -  that’s 6× faster than the industry standard, making SimpleStep Ignite ™ the fastest chemiluminescent ELISA on the market.

Our SimpleStep Ignite™ ELISA kits are made with optimized  recombinant antibody pairs  and validated in biological samples, including serum, plasma, cell culture supernatants and CSF  to deliver consistent performance batch after batch.

How do chemiluminescent ELISA kits differ from traditional colorimetric ELISA kits?

Chemiluminescent ELISA kits use a luminescent substrate that emits light as a signal, which is then measured via a luminometer. This method generally provides higher sensitivity and a broader dynamic range compared to the colorimetric method, which relies on a chromogenic substrate and optical density measurements.

Are these kits suitable for all types of samples?

Chemiluminescent ELISA kits can be used with a variety of sample types, including serum, plasma, cell culture supernatants, and other biological fluids. However, it's important to consider a specific kit's compatibility with your sample type for optimal results.

What equipment is needed to use a chemiluminescent ELISA kit?

You will need a plate reader equipped to detect chemiluminescence.

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Részletes termékinformációt talál a gyártó weboldalán: abcam.com