Promega real-time PCR (qPCR) and RT-qPCR assays provide a choice of probe-based or dye-based qPCR methods. We also offer XpressAmp™ Reagents that are compatible for use with the GoTaq® qPCR and RT-qPCR Systems and enable direct PCR amplification from viral samples without having to perform RNA or DNA extraction.

Both probe and dye-based qPCR assay systems provide sensitive detection for reproducible and earlier quantification of low- and high-copy-number targets over a broad dynamic range, together with resistance to a wide range of PCR inhibitors. They provide sensitive detection on most real time PCR instruments and are amenable to both fast and standard cycling methods.

All real-time qPCR and RT-qPCR systems also include the convenience of room-temperature reaction set-up and easy-to-use master mix formulations.