Direct insertion of sensor/tubing (shown on incubator)

Control via Adapter Plate (shown on BioSpherix C-Shuttle)

Installation

1. Set ProOx P360 on or near host chamber and plug it in.

2. Hook up sensor and gas infusion tube and insert both into the chamber.

3. Hook up gas supply.

How It Works

From outside the host chamber, the ProOx P360 works by remotely sensing the oxygen concentration inside the chamber and infusing either nitrogen to lower it or oxygen to raise it.

Installation is easy. Simply insert sensor and nozzle of infusion tube into chamber through any convenient passageway. Doors, windows, sample ports, access ports, holes (built-in or custom-drilled) all work.

Operation

FRONT PANEL INTERFACE

All operations are conveniently located at the front of the panel and all connections are located at the back and out of the way allowing for less interference and increasing usability for cell culture research and development. O2 concentration sensor readings are displayed in bright green numbers to be continuously and easily monitored from across the lab. Manual switch for gas provides a convenient shut off thus saving gas when chamber door is opened. An alarm buzzer, also manual, will remind you if you have forgotten to turn the gas back on. Bleed valve on front panel for easy calibration (top right).

Gas

USE ANY GAS SUPPLY

Conveniently utilizes gas from any source. Best supply depends on consumption. Compressed gas is best in low consumption applications. Generator is best in high consumption applications. Liquid is best in between.

SAVES GAS AND MONEY

Chamber gas consumption varies widely, but in every case the ProOx P360 always and absolutely minimizes gas consumption. It’s maximally efficient! Gas costs are reduced to absolute minimum.

1. No control yet. Chamber oxygen is ambient air oxygen (21%).

2. Control starts. Control gas is nitrogen. Infusion immediately starts to push oxygen down.

3. Oxygen is taken to setpoint. How fast depends on infusion rate. Infusion rate is adjustable. The higher the infusion rate, the faster to setpoint. Gas consumption in this phase is mostly a function of chamber size. The larger the chamber, the more gas it takes.

4. Steady-state control at setpoint is established. Infusion of control gas exactly matches chamber leakage to hold oxygen level constant. Gas consumption here is mostly a function of chamber leakage. The leakier the chamber, the more gas it takes.

5. Door of chamber is suddenly opened and closed. Steady-state is disrupted. Inrush of air is immediately detected and chamber oxygen is promptly returned to setpoint. Gas consumption depends on how wide and how long the door was opened. The more it’s disrupted, the more gas it takes to get back to setpoint.

6. Door suddenly unlatches. Leakage goes up but ProOx P360 compensates and immediately re-establishes steady state at setpoint. Gas consumption goes up dramatically, but oxygen is kept at setpoint.

7. Door gets shut tight. Leakage goes back down to normal. ProOx P360 compensates by drifting back to setpoint and reestablishes steady-state at setpoint. Gas consumption goes back down to normal levels, while oxygen stays put.

NORMOBARIC OXYGEN FEEDBACK CONTROL

ProOx P360 works in semi-sealable chambers. It controls chamber oxygen by forced displacement of gas inside chamber via dilution with either oxygen-rich or oxygenpoor control gas. Pressure inside the chamber stays the same as pressure outside. Dilution is a logarithmic process. The further away from ambient, the slower it goes. But closed-loop control is efficient. By constantly monitoring chamber oxygen, it promptly gets to setpoint. It responds immediately to correct any disturbance. It automatically adjusts to different loads. And in the process, it uses the least amount of gas possible!

Power is a function of infusion rate of control gas. The higher the infusion rate, the faster to setpoint. Above: infusion rate #4>#3>#2>#1. Infusion rate is a function of control gas supply Maximum 40 PSIG provides up to 140 SCFH.

CONTROL GAS IS NITROGEN

Setpoints are 0.1-20.7% oxygen. The lower the set point, the more time and nitrogen it takes to reach and hold the setpoint.

CONTROL GAS IS OXYGEN

Setpoints are 20.9-99.9% oxygen. The higher the set point, the more time and oxygen it takes to reach and hold the setpoint.